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There has been a remarkable transition from PCR to DNA microarray technology. Specifically, two new methods have become the basis of the most important applications of this technology: the creation of comparative genomic hybridization (CGH) and high-throughput expression analysis (HT-EA). The latter is now the most popular of these new methods in the community of molecular biologists and clinicians. The application of CGH for the detection of chromosomal imbalances has been the most common application to date. Currently, a number of commercial kits are available for CGH but they have relatively limited resolution. The high-resolution CGH method is based on the use of microarrays. Recent advances in the design of oligonucleotide microarrays and their implementation in automated microarray production have made high-resolution DNA microarray technology feasible for wide-scale use. However, these procedures are time-consuming. As such, it is essential to establish a suitable procedure in each laboratory for the construction of microarrays and their use in analysis. These procedures are not restricted to the construction of microarrays. It is also important to establish a suitable procedure for analysing and interpreting DNA microarray data. This article reviews recent developments in DNA microarrays and provides an overview of their applications in different scientific fields.Effect of temperature on the photochemical transformation of 3-nitrostyrene into 3-vinylbenzoic
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